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安全 · 高敏 · 便捷——GelRed®核酸染料（貨號(hào)41003）多維度解析！?
?? 產(chǎn)品詳細(xì)介紹
GelRed® Nucleic Acid Gel Stain（貨號(hào)41003） 是Biotium公司推出的革命性核酸染色試劑，專(zhuān)為瓊脂糖和聚丙烯酰胺凝膠中的DNA/RNA檢測(cè)設(shè)計(jì)。
- 規(guī)格：10000× 水溶液（0.5mL/1mL），兼容各類(lèi)電泳設(shè)備。
- 工作原理：通過(guò)嵌入雙鏈核酸后發(fā)射熒光（激發(fā)最大值：~300/495nm，發(fā)射最大值：~600nm），與EB（溴化乙錠）相似但更安全。
- 安全性認(rèn)證：經(jīng)Ames測(cè)試驗(yàn)證無(wú)誘變性，可通過(guò)廢棄物常規(guī)處理流程（如活性炭/漂白劑降解）。
- 兼容性：適用于紫外（UV）或藍(lán)光透射儀（470-520nm），且與TAE、TBE等緩沖液兼容。
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? 核心優(yōu)勢(shì)
1. ?? 安全性高
- 零致癌風(fēng)險(xiǎn)：不穿透細(xì)胞膜，無(wú)法進(jìn)入生物體，安全性遠(yuǎn)高于EB（IARC 1類(lèi)致癌物）！
- 廢棄物直排：實(shí)驗(yàn)廢液無(wú)需特殊處理（注：需符合當(dāng)?shù)丨h(huán)保法規(guī)）。
2. ?? 超高靈敏度
- 檢測(cè)限低至0.1ng DNA/條帶，靈敏度與EB相當(dāng)，遠(yuǎn)超SYBR® Green I！
- 熒光信號(hào)穩(wěn)定＞12小時(shí)，無(wú)需脫色，結(jié)果清晰無(wú)背景干擾。
3. ?? 操作便捷
- 即用型溶液：直接加入熔融瓊脂糖（55-60℃）中混勻，或電泳后浸泡染色（推薦濃度：1×）。
- 節(jié)省時(shí)間：染色3-5分鐘即可成像，比EB快50%！
? 購(gòu)買(mǎi)高頻疑問(wèn)Q&A
Q1：GelRed®溶劑是水還是DMSO？
答：貨號(hào)41003為水溶液，無(wú)需稀釋?zhuān)苯邮褂酶踩?nbsp;
Q2：能用于＜2%的高濃度膠嗎？
答：兼容！1-4%瓊脂糖凝膠均可使用，染色均勻無(wú)沉淀。
Q3：工作濃度是多少？
答：推薦1×濃度：每100mL膠加10μL原液（10000×），成本僅需幾毛錢(qián)/次！
Q4：藍(lán)光 vs 紫外激發(fā)，哪個(gè)更好？
答：藍(lán)光激發(fā)更優(yōu)！減少DNA損傷，且信號(hào)強(qiáng)度提升20%（配橙/紅色濾光片）。
Q5：可否用于預(yù)制膠（pre-cast gel）？
答： 避光4℃儲(chǔ)存，穩(wěn)定性＞2年；避免反復(fù)凍融！
（注：本文內(nèi)容基于品牌公開(kāi)資料及行業(yè)常規(guī)信息整理，具體以品牌信息文檔為準(zhǔn)。）
?Biotium熱賣(mài)產(chǎn)品
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貨號(hào) | 品名 | 規(guī)格 | 品牌 |
10030 | FDP (high purity) | 5 mg | Biotium |
20015 | CF®488ADonkeyAnti-RabbitIgG(H+L),HighlyCross-Adsorbed,2mg/mL | 500uL | Biotium |
20092 | Donkey Anti-Rat IgG (H+L), Highly Cross-Adsorbed | 0.5mL | Biotium |
20282 | CF®647 Goat Anti-Rabbit IgG (H+L), Highly Cross-Adsorbed, 2 mg/mL | 500uL | Biotium |
20302 | Goat Anti-Mouse IgG (H+L), Highly Cross-Adsorbed (Min X Rat) | 0.5mL | Biotium |
20432 | CF®488A Rabbit Anti-FLAG | 100 uL | Biotium |
22003 | Mini Cell Scrapers | 200ST | Biotium |
22010 | 10XFishGelatinBlockingAgent | 100ML | Biotium |
23001 | EverBrite™ Mounting Medium | 1ml | Biotium |
23005 | CoverGrip™CoverslipSealant | 15mL | Biotium |
23007 | TrueBlack® Lipofuscin Autofluorescence Quencher, 20X in DMF | 1mL | Biotium |
23012 | TrueBlack® IF Background Suppressor System (Permeabilizing) | 200 assays | Biotium |
29022 | WheatGermAgglutinin,CF®488AConjugate | 5x1mg | Biotium |
29050 | Cheetah™ Hot Start Taq DNA Polymerase | 500EU | Biotium |
29051 | EvaEZ™ Fluorometric Polymerase Activity Assay Kit | 2 x 1 mL | Biotium |
29052 | ROX Passive Reference Dye | 5ml | Biotium |
30023 | CellBrite™RedCytoplasmicMembraneDye | 1ML | Biotium |
30024 | CellBrite™ Blue Cytoplasmic Membrane-Labeling Kit | 1KIT | Biotium |
30029 | NucView® 488 Caspase 3 Assay Kit | 1KIT | Biotium |
30072 | NucView® 488 and RedDot™ 2 Apoptosis & Necrosis Kit (100 assays) | 1KIT | Biotium |
30089 | CellBrite™ Fix 640 Membrane Labeling Kit | 1KIT | Biotium |
30090 | CellBrite™ Fix 488 Membrane Labeling Kit | 1KIT | Biotium |
31000 | EvaGreen®, 20X in Water, 5x1 mL | 5x1ml | Biotium |
31019 | EvaGreen®, 2000X in DMSO | 50uL | Biotium |
31038 | PMA Enhancer for Gram Negative Bacteria, 5X Solution | 16 mL | Biotium |
31075 | Viability PCR Starter Kit with PMA | 1kit | Biotium |
31077 | EvaGreen® Plus, 20X in Water, 5x1 mL | 5ml | Biotium |
40002 | dUTP CF® Dye Conjugates | 25 nmol | Biotium |
40008 | CF®488A-dUTP,LyophilizedPowder | 25nmol | Biotium |
40012 | DMAO, 2mM in DMSO | 1ml | Biotium |
40013 | PMA (Propidium Monoazide) | 1mg | Biotium |
40019 | PMA (Propidium Monoazide) | 100uL | Biotium |
40021 | 5-Aminoallyl-UTP, Sodium Salt, 10 mM in TE Buffer | 100ul | Biotium |
40027 | dCTP CF® Dye Conjugates | 25 nmol | Biotium |
40035 | Biotin-11-CTP, 10 mM Solution | 25uL | Biotium |
40050 | Ethidium homodimer III | 1mg | Biotium |
40061 | RedDot™2 Far-Red Nuclear Stain, 200X in DMSO | 250ul | Biotium |
40069 | PMAxx™ Dye, 20 mM in H2O | 100ul | Biotium |
41001 | GelRed® Nucleic Acid Gel Stain, 3X in Water | 4L | Biotium |
41002 | GelRed® Nucleic Acid Gel Stain 10.000X in DMSO | 0.5ML | Biotium |
41003 | GelRed® Nucleic Acid Gel Stain | 0.5ml | Biotium |
41004 | GelGreen® 10,000X in DMSO | 0.5 mL | Biotium |
41005 | GelGreen® Nucleic Acid Gel Stain, 10,000X in Water | 0.5ML | Biotium |
80014 | Hydroxystilbamidine (Fluoro-Gold™) | 10mg | Biotium |
80110 | CF®488A-Dextran 10,000 MW, Anionic and Fixable | 1MG | Biotium |
80112 | CF®555-Dextran10,000MW,AnionicandFixable | 1MG | Biotium |
90066 | Biotin-XX MTSEA | 10mg | Biotium |
91020 | MTSES (2-Sulfonatoethyl methanethiosulfonate sodium salt) is a negatively charged MTS reagent. | 100mg | Biotium |
92120 | CF®488A,succinimidylester | 1UMOL | Biotium |
#70037 | SCAS | 100 mg | Biotium |
00005-100ug | α-Bungarotoxin, CF® Dye and Other Conjugates | 100ug | Biotium |
00010-1 | Alpha-Bungarotoxin | 1MG | Biotium |
00034-T | Phalloidin,CF®405M | 50U | Biotium |
00042-T | Phalloidin, CF®488A | 50U | Biotium |
00044-T | phalloidin, CF568 | 50U | Biotium |
10110-50uG | Coelenterazine (ALSO KNOWN AS NATIVE COE | 50uG | Biotium |
10111-50uG | Coelenterazine H | 50uG | Biotium |
10125-50uG | Coelenterazine 400A (DEEPBLUEC, A TRADEM | 50uG | Biotium |
20022-1mg | CF®488A Goat Anti-Human IgG (H+L), Highly Cross-Adsorbed | 1mg | Biotium |
20071-1 | CF®488A Rabbit Anti-Human IgG (H+L), Highly Cross-Adsorbed, 2 mg/mL | 50ul | Biotium |
20074-1mg | CF488A 驢 抗 人 IgG (H+L), highly cross-adsorbed | 1mg | Biotium |
20104-1 | CF®568 Goat Anti-Chicken IgY (IgG) (H+L), Highly Cross-Adsorbed, 2 mg/mL | 50ul | Biotium |
20813-50uL | Goat Anti-Rabbit IgG (H+L), Highly Cross-Adsorbed, CF® Dye Conjugates, Single Label for STORM | 50uL | Biotium |
21003-1L | One-Step Blue Protein Gel Stain, 1X | 1L | Biotium |
21004-1L | One-Step Lumitein™ Protein Gel Stain, 1X | 1L | Biotium |
21005-1L | One-Step Blue® Protein Gel Stain | 1L | Biotium |
23005-1 | CoverGrip™ Coverslip Sealant Refill | 100ml | Biotium |
23014-T | TrueBlack® Plus Lipofuscin Autofluorescence Quencher, 40X in DMSO | 50ul | Biotium |
29022-1 | WheatGermAgglutinin,CF®488AConjugate | 1mg | Biotium |
29023-1 | WheatGermAgglutinin,CF®594Conjugate | 1mg | Biotium |
29026-1 | CF®640R WGA | 1mg | Biotium |
29027-1 | CF®405S WGA CF®405S 404/431 nm | 1mg | Biotium |
29028-1 | WheatGermAgglutinin,CF®405MConjugate | 1mg | Biotium |
29091-10mg | R-Phycoerythrin (RPE), lyophilized solid, ammonium sulfate free | 10mg | Biotium |
29092-10mg | Allophycocyanin (APC), cross-linked lyophilized solid, ammonium sulfate free | 10mg | Biotium |
30020-T | ATP-Glo™ Bioluminometric Cell Viability Assay Kit (50 assays) | 1KIT | Biotium |
30028-L3 | Steady-Luc™ Firefly HTS Assay Kit (Lyophilized) 10 x 1000 Assays | 10000TST | Biotium |
30029-T | NUCVIEW 488 CASPASE 3 ASSAY KIT, TRIAL SIZE 1ST | 1ST | Biotium |
30089-T | CellBrite™ Fix 640 Membrane Labeling Kit, Trial Size | 1KIT | Biotium |
30090-T | CellBrite™ Fix 488 Membrane Labeling Kit, Trial Size | 1KIT | Biotium |
30096-T | MemBrite™ Fix 594/615 Cell Surface Staining Kit, 100 Reactions | 1KIT | Biotium |
30114-T | ExoBrite™ 640/660 EV Membrane Staining Kit, 100 labelings | 1KIT | Biotium |
31000-T | EvaGreen® Dye, 20X in Water | 1mL | Biotium |
31003-1 | FastEvaGreen®MasterMixforqPCR(500rxn) | 5x1ML | Biotium |
31028-T | AccuClear® Ultra High Sensitivity dsDNA Quantitation Kit with DNA Standard, trial size (200 assays) | 1kit | Biotium |
31041-1 | Forget-Me-Not™ EvaGreen® qPCR Master Mix (500 reactions) | 5x1ml | Biotium |
31069-T | AccuGreen™ Broad Range dsDNA Quantitation Kit (100 assays) | 1kit | Biotium |
31077-T | EvaGreen®Plus,20XinWater,1mL | 1ML | Biotium |
33021-1 | GloMelt™ Thermal Shift Protein Stability kit | 1kit | Biotium |
33021-T | GloMelt™ Thermal Shift Protein Stability kit | 1kit | Biotium |
33022-1 | GloMelt™ Thermal Shift Protein Stability kit | 1kit | Biotium |
33022-T | GloMelt™ Thermal Shift Protein Stability kit | 1kit | Biotium |
40002-T | dUTP CF® Dye Conjugates | 5 nmol | Biotium |
40020-1 | 5-Aminoallyl-dUTP (AA-dUTP), Lyophilized Powder | 1 mg | Biotium |
40027-T | dCTP CF® Dye Conjugates | 5 nmol | Biotium |
40060-T | RedDot™1 Far-Red Nuclear Stain, 200X in Water | 25uL | Biotium |
41010-1mL | 6X GelRed Prestain Buffer with Orange Tracking Dye | 1mL | Biotium |
41029-50G | GelRed®AgaroseLE | 50G | Biotium |
90066-1 | MTSEA Biotin-XX | 50mg | Biotium |
E90002 | PMA-Lite™ LED Photolysis Device | 1EA | Biotium |
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