手機(jī)版
化工儀器網(wǎng)手機(jī)版
化工儀器網(wǎng)小程序
官方微信
公眾號(hào)：chem17
掃碼關(guān)注視頻號(hào)
| Synonyms : | Shh, HHG-1 | ||||||||||||||||
| Description : | Hedgehog （Hh） proteins are widely distributed throughout the animal kingdom and play important regulatory roles in various development processes. The three known mammalian Hh proteins, Sonic （Shh）, Desert （Dhh） and Indian （Ihh） are structurally related and share a high degree of amino acid sequence identity （e.g. Shh and Ihh are 93 % identical）. Although, Hh proteins have unique expression patterns and distinct biological roles within their respective regions of secretion, they signal through the same Patched （Ptc）/Smoothened （Smo） receptor complex, activate the same intracellular signaling pathway, and can substitute for each other in experimental systems. The mature biologically active form of Hh molecules is produced by autocatalytic cleavage of their precursor proteins and corresponds to approximay the N-terminal half of the precursor molecule. Recombinant human Shh is a 20.6 kDa protein consisting of 174 amino acid residues, which correspond to the active N-terminal portion of the Shh precursor. | ||||||||||||||||
| Catalog #: | 100-45B | ||||||||||||||||
| Source : | （BTI-Tn-5B1-4） High-5 Insect Cells* | ||||||||||||||||
| Stability : | The lyophilized protein is stable for at least 2 years from date of receipt at -200C. Reconstituted Shh is stable for at least 3 months when stored in working aliquots with a carrier protein at -200C. Avoid repeated freeze/thaw cycles.3 | ||||||||||||||||
| Purity : | Greater than 98% by SDS-PAGE gel and HPLC analyses. | ||||||||||||||||
| Endotoxin Level : | Endotoxin level is less than 0.1 ng per μg （1EU/μg）. | ||||||||||||||||
| Biological Activity : | Determined by its ability to induce alkaline phosphatase production by C3H/10T1/2 （CCL-226） cells. The expected ED50 for this effect is 0.8-1.0 μg/ml. | ||||||||||||||||
| PubMed Link : | Sonic+AND+Hedgehog | ||||||||||||||||
| Country of Origin : | USA | ||||||||||||||||
| | | ||||||||||||||||
| |
| ||||||||||||||||
相關(guān)產(chǎn)品
免責(zé)聲明
- 凡本網(wǎng)注明“來(lái)源：化工儀器網(wǎng)”的所有作品，均為浙江興旺寶明通網(wǎng)絡(luò)有限公司-化工儀器網(wǎng)合法擁有版權(quán)或有權(quán)使用的作品，未經(jīng)本網(wǎng)授權(quán)不得轉(zhuǎn)載、摘編或利用其它方式使用上述作品。已經(jīng)本網(wǎng)授權(quán)使用作品的，應(yīng)在授權(quán)范圍內(nèi)使用，并注明“來(lái)源：化工儀器網(wǎng)”。違反上述聲明者，本網(wǎng)將追究其相關(guān)法律責(zé)任。
- 本網(wǎng)轉(zhuǎn)載并注明自其他來(lái)源（非化工儀器網(wǎng)）的作品，目的在于傳遞更多信息，并不代表本網(wǎng)贊同其觀點(diǎn)和對(duì)其真實(shí)性負(fù)責(zé)，不承擔(dān)此類(lèi)作品侵權(quán)行為的直接責(zé)任及連帶責(zé)任。其他媒體、網(wǎng)站或個(gè)人從本網(wǎng)轉(zhuǎn)載時(shí)，必須保留本網(wǎng)注明的作品第一來(lái)源，并自負(fù)版權(quán)等法律責(zé)任。
- 如涉及作品內(nèi)容、版權(quán)等問(wèn)題，請(qǐng)?jiān)谧髌钒l(fā)表之日起一周內(nèi)與本網(wǎng)聯(lián)系，否則視為放棄相關(guān)權(quán)利。
采購(gòu)中心