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Plant Phosphatidic acid ELISA kit
INTENDED USE
The PA ELISA is to be used for the quantitative determination of PA. The assay will recognize both natural and recombinant PA. The kit has been configured for research use only and is not to be used in diagnostic procedures.
PRINCIPLE OF THE METHOD
The PA kit is a solid phase phase sandwich enzyme linked immuno sorbent assay（ELISA）. Samples , including standards of known PA concentrations and unknowns are pipetted into these wells. During the first incubation, the PAantigen and a biotinylated monoclonal antibody specific for PAare simultaneously incubated. After washing, the enzyme（streptavidin-peroxydase）is added. After incubation and washing to remove all the unbound enzyme, a substrate solution which is acting on the bound enzyme is added to induce a coloured reaction product. The intensity of this coloured product is directly proportional to the concentration of PApresent in the samples.
REAGENTS PROVIDED AND RECONSTTTUTION
| REAGENTS（Store at 2-8℃） | 1×96 WELLS | 0.5×96 WELLS | RECONSTTTUTION |
| 96/48-wells microtiter plates | 1 | 0.5 | Ready-to-use |
| Plastiv cover | 2 | 1 | Ready-to-use |
| Standard: 40ng/ml | 1Vials （0.6ml） | 0.5Vials （0.3ml） | See reagents preparation on page 3 |
| Blank control | 1Vials （1.0ml） | 1Vials （0.5ml） | Ready-to-use |
| Standard Diluent | 1Vials （5ml） | 1Vials （2.5ml） | Ready-to-use |
| Biotinylated anti-PA | 1Vials （6ml） | 1Vials （3.0ml） | Ready-to-use |
| Streptavidin-HRP | 1Vials （10ml） | 1Vials （5.0ml） | Ready-to-use |
| Washing Buffer | 1Vials （60ml） | 1Vials （30ml） | 20× concentrate |
| Substrate A | 1Vials （6.0ml） | 1Vials （3.0ml） | Ready-to-use |
| Substrate B | 1Vials 6.0ml） | 1Vials （3.0ml） | Ready-to-use |
| Stopping Solution | 1Vials （6.0ml） | 1Vials （3.0ml） | Ready-to-use |
MATERIAL REQUIRED BUT NOT PROVIDED
l Distilled water
l Pipettes:10ul、50ul、100ul、200ul、1000ul。
l Vortex mixer and magnetic stirrer.
INTENDED USE
The PA ELISA is to be used for the quantitative determination of PA. The assay will recognize both natural and recombinant PA. The kit has been configured for research use only and is not to be used in diagnostic procedures.
PRINCIPLE OF THE METHOD
The PA kit is a solid phase phase sandwich enzyme linked immuno sorbent assay（ELISA）. Samples , including standards of known PA concentrations and unknowns are pipetted into these wells. During the first incubation, the PAantigen and a biotinylated monoclonal antibody specific for PAare simultaneously incubated. After washing, the enzyme（streptavidin-peroxydase）is added. After incubation and washing to remove all the unbound enzyme, a substrate solution which is acting on the bound enzyme is added to induce a coloured reaction product. The intensity of this coloured product is directly proportional to the concentration of PApresent in the samples.
REAGENTS PROVIDED AND RECONSTTTUTION
| REAGENTS（Store at 2-8℃） | 1×96 WELLS | 0.5×96 WELLS | RECONSTTTUTION |
| 96/48-wells microtiter plates | 1 | 0.5 | Ready-to-use |
| Plastiv cover | 2 | 1 | Ready-to-use |
| Standard: 40ng/ml | 1Vials （0.6ml） | 0.5Vials （0.3ml） | See reagents preparation on page 3 |
| Blank control | 1Vials （1.0ml） | 1Vials （0.5ml） | Ready-to-use |
| Standard Diluent | 1Vials （5ml） | 1Vials （2.5ml） | Ready-to-use |
| Biotinylated anti-PA | 1Vials （6ml） | 1Vials （3.0ml） | Ready-to-use |
| Streptavidin-HRP | 1Vials （10ml） | 1Vials （5.0ml） | Ready-to-use |
| Washing Buffer | 1Vials （60ml） | 1Vials （30ml） | 20× concentrate |
| Substrate A | 1Vials （6.0ml） | 1Vials （3.0ml） | Ready-to-use |
| Substrate B | 1Vials 6.0ml） | 1Vials （3.0ml） | Ready-to-use |
| Stopping Solution | 1Vials （6.0ml） | 1Vials （3.0ml） | Ready-to-use |
MATERIAL REQUIRED BUT NOT PROVIDED
l Distilled water
l Pipettes:10ul、50ul、100ul、200ul、1000ul。
l Vortex mixer and magnetic stirrer.
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