国产精品视频一区二区三区四,亚洲av美洲av综合av,99国内精品久久久久久久,欧美电影一区二区三区电影

產(chǎn)品推薦:氣相|液相|光譜|質(zhì)譜|電化學(xué)|元素分析|水分測定儀|樣品前處理|試驗機|培養(yǎng)箱


化工儀器網(wǎng)>技術(shù)中心>其他文章>正文

歡迎聯(lián)系我

有什么可以幫您? 在線咨詢

測量應(yīng)用案例-38-90Plus

來源:美國布魯克海文儀器公司   2015年06月08日 09:41  
 文獻名: Effects of cell-penetrating peptides on transduction efficiency of PEGylated adenovirus
 
作者: Adane S. Nigatu, Sravanthi Vupputuri, Nicholas Flynn, Joshua D. Ramsey
School of Chemical Engineering, Oklahoma State University, Stillwater, OK 74078, USA
 
摘要:
Background
Adenovirus (Ad) is one of the viral vectors most widely used for gene delivery. The virus, however, has serious shortcomings such as immunogenicity, promiscuous tropism, and the inability to efficiently infect certain types of cells. The goal of this study was to improve the ability of an Ad-based vector to efficiently transform cells that lack the native coxsackie-adenovirus receptor (CAR−) by modifying the virus with CPP-PEG conjugates.
 
Methods
The vector was produced by PEGylating Ad, which packages a lacZ reporter gene, and then conjugating CPPs to form CPP-PEG-Ad particles. The study compared the effectiveness of four different CPPs: Pen, Tat, Pep1, and pArg. The effects of CPP amount per virus, degree of PEGylation, and PEG molecular weight on transduction efficiency were studied on CAR− NIH/3T3 cells.
 
Results
CPP-PEG-Ad particles transduced CAR− cells significantly better than unmodified Ad. Pen, the most effective CPP, produced an 80-fold improvement in transduction compared to the unmodified virus. The Pen peptide utilized a combination of electrostatic and hydrophobic interactions with the cell membrane to maximize cellular association while the other CPPs used only electrostatic or hydrophobic interactions but not both. Lastly, higher degrees of PEGylation, which prompted PEG to adopt a “brush” conformation, resulted in more efficient CPP-PEG-Ad particles because of both better conjugation of CPPs to the PEGylated virus and better exposure of the conjugated CPPs on the surface of the particle.
 
Conclusions
CPP-PEG-Ad particles efficiently deliver genes to cells that Ad alone would not efficiently infect, thereby extending potential gene therapy treatments to a much broader range of cell types and diseases.
 
關(guān)鍵詞:Gene vector; Adenovirus; Cell-penetrating peptides; PEGylation; Coxsackie virus and adenovirus receptor

免責(zé)聲明

  • 凡本網(wǎng)注明“來源:化工儀器網(wǎng)”的所有作品,均為浙江興旺寶明通網(wǎng)絡(luò)有限公司-化工儀器網(wǎng)合法擁有版權(quán)或有權(quán)使用的作品,未經(jīng)本網(wǎng)授權(quán)不得轉(zhuǎn)載、摘編或利用其它方式使用上述作品。已經(jīng)本網(wǎng)授權(quán)使用作品的,應(yīng)在授權(quán)范圍內(nèi)使用,并注明“來源:化工儀器網(wǎng)”。違反上述聲明者,本網(wǎng)將追究其相關(guān)法律責(zé)任。
  • 本網(wǎng)轉(zhuǎn)載并注明自其他來源(非化工儀器網(wǎng))的作品,目的在于傳遞更多信息,并不代表本網(wǎng)贊同其觀點和對其真實性負責(zé),不承擔(dān)此類作品侵權(quán)行為的直接責(zé)任及連帶責(zé)任。其他媒體、網(wǎng)站或個人從本網(wǎng)轉(zhuǎn)載時,必須保留本網(wǎng)注明的作品第一來源,并自負版權(quán)等法律責(zé)任。
  • 如涉及作品內(nèi)容、版權(quán)等問題,請在作品發(fā)表之日起一周內(nèi)與本網(wǎng)聯(lián)系,否則視為放棄相關(guān)權(quán)利。
企業(yè)未開通此功能
詳詢客服 : 0571-87858618
阳朔县| 万州区| 苍山县| 威宁| 定结县| 永济市| 喀什市| 鹤山市| 黄浦区| 南岸区| 涿鹿县| 大方县| 乌兰浩特市| 河南省| 溧阳市| 临泉县| 自贡市| 克拉玛依市| 八宿县| 景东| 赤峰市| 沂源县| 凤冈县| 惠水县| 台湾省| 宁阳县| 长葛市| 扶沟县| 岳西县| 大洼县| 盘锦市| 平山县| 中山市| 荥阳市| 淮安市| 天祝| 沾化县| 阿拉尔市| 明溪县| 永寿县| 建阳市|