【文獻標題】Proanthocyanidins and β?Glucan Synergistically Regulate Intestinal Inflammation in Dextran Sulfate Sodium-Induced Colitis Mice

【作者】Pengkui Xia, Ruyi Li, Mianhong Chen et.al

【作者單位】Huazhong Agricultural University（華中農(nóng)業(yè)大學）

【文獻中引用產(chǎn)品】

小鼠白介素6(IL-6)ELISA試劑盒

小鼠白介素1β (IL-1β)ELISA試劑盒

小鼠白介素10(IL-10)ELISA試劑盒

小鼠腫瘤壞死因子α(TNF-α)ELISA試劑盒

【關鍵詞】proanthocyanidins, β-glucan, colitis, synergistic anti-inflammatory function

【DOI】doi.org/10.1021/acs.jafc.4c03544

【影響因子(IF)】5.69

【出版期刊】《Journal of Agricultural and Food Chemistry》

【產(chǎn)品原文引用】

Biomarker Analysis. Biomarker levels in the colonic tissueswere analyzed. Briefly, 100 mg of colon or liver tissue samples was weighted and homogenized in a precooled phosphate buffered solution (PBS, 1:9 [w/v]). The homogenate samples were centrifuged at 10,000g for 10 min at 4 °C, and the supernatant was collected. The protein concentration of the supernatant was determined by Bicinchoninic acid detection kits (Biosharp Biotechnology Co., Ltd.,Hefei, China). The contents of interleukin (IL)-6, IL-1β, IL-10, andtumor necrosis factor (TNF)-α in the colon were assayed using enzyme-linked immunosorbent assay (ELISA) kits (Shanghai Huding Biotechnology Co., Ltd., Shanghai, China). 

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