A synthetic peptide corresponding to residues following the cleavage site of human PARP-1 was used as immunogen. The antibody only recognize p85 cleaved-form of PARP-1.

50 mM Tris-Glycine （pH 7.4）, 0.15 M NaCl, 40% Glycerol, 0.01% sodium azide and 0.05% BSA.

PARP1, ADPRT, PPOL, Poly [ADP-ribose] polymerase 1, ADPRT;NAD（+） ADP-ribosyltransferase 1;Poly[ADP-ribose] synthetase 1

1. Ikejima, M, et al. The zinc fingers of human poly（ADP-ribose） polymerase are differentially required for the recognition of DNA breaks and nicks and the consequent enzyme activation. Other structures recognize intact DNA. J.Biol.Chem. 265: 21907
2. Lazebnik, Y.A., et al. Cleavage of poly（ADP-ribose） polymerase by a proteinase with properties like ICE. Nature 371: 346
3. Tewari, M, et al. Yama/CPP32 beta, a mammalian homolog of CED-3, is a CrmA-inhibitable protease that cleaves the death substrate poly（ADP-ribose） polymerase. Cell 81: 801
4. Kaufmann, S.H., et al. Specific proteolytic cleavage of poly（ADP-ribose） polymerase: An early marker of chemotherapy-induced apoptosis. Cancer Res. 53: 2976
 

1. Yoon S, Molloy MJ, Wu MP, et al, C6ORF32 is upregulated during muscle cell differentiation and induces the formation of cellular filopodia ,Developmental Biology 301 （2007） 70-81
[Application: ICC ]
2. Shangary S, Ding K, Qiu S, et al, Reactivation of p53 by a specific MDM2 antagonist （MI-43） leads to p21-mediated cell cycle arrest and selective cell death in colon cancer ,Mol. Cancer Ther. 7 （2008） 1533 - 1542
[Application: WB ]