APC anti-mouse TER-119/Erythroid Cells Biolegend
參考價(jià) | ¥ 1040 |
訂貨量 | ≥1 |
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APC anti-mouse TER-119/Erythroid Cells具體參數(shù)>>>
Clone | TER-119 |
Isotype | Rat IgG2b, κ |
Reactivity | Mouse |
Immunogen | Day-14 fetal liver cells from a C57BL/6 mouse |
Formulation | Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide. |
Preparation | The antibody was purified by affinity chromatography, and conjugated with APC under optimal conditions. The solution is free of unconjugated APC and unconjugated antibody. |
Concentration | 0.2mg/ml |
Recommended Usage | Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For immunofluorescent staining, the suggested use of this reagent is ≤1.0 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application. |
Applications | FC - Quality tested |
Application Notes | The TER-119 antibody is useful for distinguishing erythrocytes and cells in the erythroid lineage. Additional reported applications (for the relevant formats) include: immunoprecipitation1, Western blotting1, complement-mediated cytotoxicity3, and immunohistochemical staining of acetone-fixed frozen sections and formalin-fixed paraffin-embedded sections. LEAF™ purified antibody (Endotoxin <0.1 EU/μg, Azide-Free, 0.2 μm filtered) is recommended for functional assays (Cat. No. 116214). |
Application References | 1. Kina T, et al. 2000. Br. J. Haematol. 109:280. (IP, WB) |
Description | The TER-119 antigen is a 52 kD glycophorin A-associated protein, also known as Ly-76. TER-119 is an erythroid-specific antigen expressed on early proerythroblasts to mature erythrocytes, but not on BFU-E or CFU-E. |
Storage | The antibody solution should be stored undiluted at 4°C and protected from prolonged exposure to light. Do not freeze. |
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