Sulforhodamine B sodium s Acid Red 52

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型號(hào) Sulforhodamine B sodium s
產(chǎn)地
廠商性質(zhì) 生產(chǎn)廠家
更新時(shí)間 2019/5/28 10:10:50
訪問(wèn)次數(shù) 457

產(chǎn)品標(biāo)簽

Acid Red 52 Kiton Red 620 sulforhodamine

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詳細(xì)信息 在線詢價(jià)

供貨周期	現(xiàn)貨	規(guī)格	10 mM * 1 mL ; 100 mg ; 1 g
貨號(hào)	HY-D0974

Sulforhodamine B sodium salt

產(chǎn)品活性：Sulforhodamine B sodium salt 是一種熒光染料，可用于定量測(cè)定培養(yǎng)細(xì)胞的細(xì)胞蛋白量。

研究領(lǐng)域：Others

作用靶點(diǎn)：Others

In Vitro: Sulforhodamine B (SRB) is often used as a membrane-impermeable polar tracer or used for cell density determination via determination of cellular proteins (cytotoxicity assay). The SRB assay has been used to inexpensively conduct various screening assays to investigate cytotoxicity in cell based studies. This method relies on the property of SRB, which binds stoichiometrically to proteins under mild acidic conditions and then can be extracted using basic conditions; thus, the amount of bound dye can be used as a proxy for cell mass, which can then be extrapolated to measure cell proliferation. The protocol can be divided into four main steps: preparation of treatment, incubation of cells with treatment of choice, cell fixation and SRB staining, and absorbance measurement. This assay is limited to manual or semiautomatic screening, and can be used in an efficient and sensitive manner to test chemotherapeutic drugs or small molecules in adherent cells. It also has applications in evaluating the effects of gene expression modulation (knockdown, gene expression upregulation), as well as to study the effects of miRNA replacement on cell proliferation.