ELISpot底物:用于ALP的BCIP/NBT-plus--用于利用鏈霉親和素-堿性磷酸酶（ALP）酶結(jié)合物開(kāi)發(fā)ELISpot實(shí)驗(yàn)。僅供研究使用，不適用于診斷程序。

艾美捷ELISpot底物:用于ALP的BCIP/NBT-plus：

貨號(hào)：MBT-3650-10

應(yīng)用：ELISpot

產(chǎn)品內(nèi)容：BCIP/NBT-plus底物（用于ELISpot）

運(yùn)輸條件：常溫運(yùn)輸

儲(chǔ)存條件：收到后請(qǐng)?jiān)?span style="font-family: Calibri;">4-8℃保存

有效期：自收到之日起至少18個(gè)月

推薦使用方法：使用前請(qǐng)將底物恢復(fù)至室溫。將底物溶液通過(guò)0.45微米濾膜過(guò)濾后，加入經(jīng)過(guò)堿性磷酸酶孵育和隨后洗滌的ELISpot板中。顯色至斑點(diǎn)出現(xiàn)為止。通過(guò)用大量自來(lái)水沖洗以終止顏色反應(yīng)。

部分文獻(xiàn)參考：

Kim et al,Specific targeting of cancer vaccines to antigen-presenting cells via an endogenous TLR2/6 ligand derived fromcysteinyl-tRNA synthetase 1.Molecular therapy:the journal of the American Society of Gene Therapy 2024

Nguyen et al,Lipid nanoparticle-encapsulated DNA vaccine confers protection against swine and human-origin H1N1influenza viruses mSphere 2024

Mietz et al.,Human effectorCD8+T cells with an activated and exhausted-like phenotype control tumour growth in vivo in ahumanized tumour model.EBioMedicine 2024

Leekha et al,Multi antigen intranasal vaccine protects against challenge with sarbecoviruses and prevents transmission inhamsters.Nature communications 2024

Lu et al,Plasmodium vivax serological exposure markers PvMSP1-42-induced humoral and memory B-cell responsegenerates long-lved antibodies.PLoS pathogens 2024