The NF-κB reporter (Luc) Jurkat cell line is designed for monitoring nuclear factor Kappa B (NF-κB) signal transduction pathways. It contains a firefly luciferase gene driven by four copies of the NF-κB response element located upstream of the minimal TATA promoter. After activation by pro-inflammatory cytokines or stimulants of lymphokine receptors, endogenous NF-κB transcription factors bind to the DNA response elements, inducing transcription of the luciferase reporter gene.

This cell line has been functionally validated and responds to phorbol 12-myristate 13-acetate (PMA) + ionomycin and TNFα. It can be used to measure TCR-mediated T cell activation through co-stimulation with anti-CD3 + anti-CD28 antibodies.

This product has been cited 6 times.

Purchase of this cell line is for research purposes only; commercial use requires a separate license. View the full terms and conditions.