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Antibody to Fibroblast Growth Factor 2, Basic (FGF2)操作規(guī)程

來(lái)源:上海武昊經(jīng)貿(mào)有限公司   2012年12月19日 17:26  

[ PRODUCT INFORMATION ]
Immunogen: FGF2
Clonality: Polyclonal
Host: Rabbit
Species Reactivity: Human
Ig type: Rabbit IgG
Purification: Antibodies are purified by target protein affinity chromatography.
Applications: WB, ICC, IHC-P, IHC-F 
Form: Liquid
Size: 100µg
[ IMMUNOGEN INFORMATION ]
Immunogen: Recombinant human FGF2 (Pro143~Ser288) expressed in E.coli.
Molecular Weight: 20.2 kDa
USCN accession No.: P90551Hu01
Sequence: The target protein is fused with two N-terminal Tags, His-tag and T7-tag and its sequence is listed below.
MGSSHHHHHHSSGLVPRGSHMASMTGGMGRGSEF-PALPEDGG SGAFPPGHFK DPKRLYCKNG GFFLRIHPDG RVDGVREKSD PHIKLQLQAE ERGVVSIKGV CANRYLAMKE DGRLLASKCV TDECFFFERL ESNNYNTYRS RKYTSWYVAL KRTGQYKLGS KTGPGQKAIL FLPMSAKS

 

[ ANTIBODY SPEFICITY ]
Anti FGF2 is a rabbit polyclonal antibody raised against human FGF2. This antibody has been selected for its ability to recognize human FGF2 in immunohistochemical staining and western blotting, non cross-reactive with other members of the family.
[ APPLICATIONS ]
Western blotting: 1:100-400
Immunocytochemistry in formalin fixed cells: 1:100-500
Immunohistochemistry in formalin fixed frozen section: 1:100-500
Immunohistochemistry in paraffin section: 1:50-200
Optimal working dilutions must be determined by end user. 
[ CONTENTS ]
Form & Buffer: Supplied as solution form in PBS, pH 7.4, containing 0.02%Na3N, 50% glycerol.
[ STORAGE ]
Store at 4oC for frequent use. Stored at -20oC to -80oC in a manual defrost freezer for one year without detectable loss of activity. Avoid repeated freeze-thaw cycles.

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