Catalog #

Product Description

ICP0383

The affinity purified rabbit polyclonal anti-acetylated lysine antibodies were developed using a unique technique that utilizes the acetylated KLH as the immunogen. The acetylated lysine-specific antibodies were specifically purified and conjugated to d-Biotin via amine acylation. Direct labelling of the primary antibody will avoid the use of secondary antibodies therefore eliminating the interference of the secondary antibody-conjugates.

Western blot analysis of the acetylated protein profile from TSA treated (A) and non-treated human melanoma cell (MMRU) lysates (B).

Species

Rabbit

Formulation

250 µg/mL antibody in PBS, pH 7, with 50% glycerol

Immunogen

Acetylated KLH conjugates

Purification

The antibody was purified on acetyl-lysine agarose.

Conjugation

Acylation with NHS-biotin; biotin/Ab molar ratio - 10:1

Specificity

The antibody recognizes proteins acetylated on lysine residues. Tested: acetylated histone, acetylated BSA, and acetylated MBP; there was no reaction to the non-acetylated proteins.

Scientific Description

DNA transcription cannot take place unless DNA is unwound from the nucleosomes. The cell unwinds the DNA by acetylating the lysine residues within the proteins. It has been suggested that acetylation of non-histone proteins (e.g. transcription factors) and histones may be involved. Acetylation of the proteins may result in signal transduction within the chromatic domains.

Storage & Stability

Product is stable for several weeks at 4°C. For extended storage,  store product at -20°C. Expiration date is one year from date of shipping if stored properly.

Product Specific References

1. 1. FEBS Lett. 2002. 513(1): 124-128. doi:10.1016/S0014-5793(01