Catalog #

Product Description

ICP0381

Affinity purified rabbit polyclonal anti-acetylated lysine antibody (anti-AcK) was developed using a unique technique that utilizes acetylated KLH as the immunogen. The purified antibody was conjugated to horseradish peroxidase (HRP) via reductive amination. Direct labelling of the primary antibody will avoid the use of secondary antibodies therefore eliminating the interference of the secondary antibody-conjugates.

Western blot analysis of acetylated histone from TSA-treated (A) and non-treated melanoma cell lysates (B) with ICP0381.

Species

Rabbit

Formulation

250 µg/mL antibody in stabilizing buffer with 50% glycerol

Immunogen

Acetylated KLH conjugates

Purification

The antibody was purified on acetyl-lysine agarose.

Conjugation

Reductive amination; HRP/Ab molar ratio - 10:1

Specificity

The antibody recognizes proteins acetylated on lysine residues. Tested: acetylated histone, acetylated BSA, and acetylated MBP; there was no reaction to the non-acetylated proteins.

Applications

For direct immunofluorescence experiments

Scientific Description

DNA transcription cannot take place unless DNA is unwound from the nucleosomes. The cell unwinds the DNA by acetylating the lysine residues within the proteins. It has been suggested that acetylation of non-histone proteins (e.g. transcription factors) and histones may be involved. Acetylation of the proteins may result in signal transduction within the chromatic domains.

Storage & Stability

Product is stable for several weeks at 4°C. For extended storage, store product at -20°C. Expiration date is one year from date of shipping if stored properly.

Product Specific References

1. 1. Curr. Opin. Chem. Biol. 1997. 1(3): 300-308. doi:10.1016/S1367-5931(97)80066-X.

2. 2. Curr. Biol. 2002.12(4): R141-R143. doi:10.1016/S0960-9822(02)00709-1.

3. 3. J. Cel. Rep. 2014. 7(5): 1471-1480. doi:10.1016/j.celrep.2014.04.021.