Flexcell細(xì)胞加壓培養(yǎng)系統(tǒng)

美國(guó)flexcell:體外細(xì)胞應(yīng)力刺激培養(yǎng)金標(biāo)準(zhǔn)

l  歷史悠久、成功使用案例和文獻(xiàn)多、無(wú)技術(shù)和使用風(fēng)險(xiǎn)：30多年歷史，6000+文獻(xiàn)，1300+實(shí)驗(yàn)室使用，國(guó)內(nèi)200+單位使用

l  真空動(dòng)力柔性基底膜細(xì)胞應(yīng)力應(yīng)變和三維細(xì)胞接種凝膠應(yīng)力應(yīng)變

l  不同基底剛度牽張：2019年研制了基底剛度1-60kPa可調(diào)控牽張拉伸培養(yǎng)皿和多孔板。

l  加載力學(xué)類型全面：牽張、壓力、流體剪切應(yīng)力

l  受力均勻、培養(yǎng)效果好：真空氣體牽張、壓縮刺激方式

l  不同基底剛度模量力學(xué)環(huán)境裝置的廠家，2019進(jìn)一步研制了 “更軟”基底剛度可調(diào)節(jié)可牽張培養(yǎng)裝置：

      0.1-80kPa可調(diào)控牽張拉伸培養(yǎng)皿和多孔板，避免細(xì)胞在體外培養(yǎng)受到“硬度”沖擊

l  模擬任意波形：動(dòng)靜、靜態(tài)以及自定義波形

l  細(xì)胞量大，便于后期分析：支持四個(gè)24孔或6孔牽張拉伸

l  即用型國(guó)際標(biāo)準(zhǔn)化的各種蛋白預(yù)先包被應(yīng)力刺激培養(yǎng)耗材

l  適用各種形態(tài)細(xì)胞組織樣品：?jiǎn)螌蛹?xì)胞、3D細(xì)胞構(gòu)建體、組織或3D聚集體

l  多模態(tài)刺激：靜態(tài)刺激、周期性動(dòng)態(tài)刺激、動(dòng)靜態(tài)對(duì)照、單軸向或雙軸向（等邊軸）牽張刺激

l  的可以動(dòng)靜態(tài)對(duì)照的廠家：可使同一塊壓力或牽張培養(yǎng)板部分孔受力。

l  的倒置和正置顯微鏡系統(tǒng)都兼容的廠家： 受力同時(shí)可實(shí)時(shí)觀察

l  整合柔性基底拓?fù)湮D案和微通道, 方便高級(jí)實(shí)驗(yàn)?zāi)Ｐ驮O(shè)計(jì)

l  整合力電刺激偶聯(lián)，方便高級(jí)實(shí)驗(yàn)?zāi)Ｐ驮O(shè)計(jì)

l  牽張、流體剪切多力混合同時(shí)加載，方便高級(jí)實(shí)驗(yàn)?zāi)Ｐ驮O(shè)計(jì)

l  使用Flexcell® Transwell Holder三維細(xì)胞（或者）拉力或壓力共培養(yǎng)

l  設(shè)備模塊化設(shè)計(jì)牽張、壓縮、流體剪切可以任意組合，緊湊大氣。

以下是一些有關(guān)細(xì)胞力學(xué)培養(yǎng)的文獻(xiàn)推薦：

- Bougault C, Aubert-Foucher E, Paumier A, Perrier-Groult E, Huot L, Hot D, Duterque-Coquillaud M, Mallein-Gerin F. Dynamic compression of chondrocyte-agarose constructs reveals new candidate mechanosensitive genes. PLoS One 7(5):e36964,2012.

- Bougault C, Paumier A, Aubert-Foucher E, Mallein-Gerin F. Molecular analysis of chondrocytes cultured in agarose in response to dynamic compression. BMC Biotechnol 8:71,2008.

- Chen X, Guo J, Yuan Y, Sun Z, Chen B, Tong X, Zhang L, Shen C, Zou J. Cyclic compression stimulates osteoblast differentiation via activation of the Wnt/β-catenin signaling pathway. Molecular Medicine Reports 15(5):2890-2896, 2017.

- Damaraju S, Matyas JR, Rancourt DE, Duncan NA. The effect of mechanical stimulation on mineralization in differentiating osteoblasts in Collagen-I scaffolds. Tissue Eng Part A 20(23-24):3142-3153, 2014.

- Damaraju S, Matyas JR, Rancourt DE, Duncan NA. The role of gap junctions and mechanical loading on mineral formation in a Collagen-I scaffold seeded with osteoprogenitor cells. Tissue Eng Part A 21(9-10):1720-32, 2015.

- Fermor B, Haribabu B, Weinberg JB, Pisetsky, Guilak F. Mechanical stress and nitric oxide influence leukotriene production in cartilage. Biochemical and Biophysical Research Communications 285:806–810,2001.

- Fermor B, Weinberg JB, Pisetsky DS, Guilak F. The influence of oxygen tension on the induction of the nitric oxide and prostaglandin E2 by mechanical stress in articular cartilage. Osteoarthritis Cartilage 13:935-941,2005.

- Fermor B, Weinberg JB, Pisetsky DS, Misukonis MA, Banes AJ, Guilak F. The effects of static and intermittent compression on nitric oxide production in articular cartilage explants. J Orthop Res 9(4):729-737, 2001.

- Fermor B, Weinberg JB, Pisetsky DS, Misukonis MA, Fink C, Guilak F. Induction of cyclooxygenase-2 by mechanical stress through a nitric oxide-regulated pathway. Osteoarthritis Cartilage 10:792–798,2002.

- Fink C, Fermor B, Weinberg JB, Pisetsky DS, Misukonis MA, Guilak F. The effect of dynamic mechanical compression on nitric oxide production in the meniscus. Osteoarthritis Cartilage 9(5):481-487, 2001.

- Fox DB, Cook JL, Kuroki K, Cockrell M. Effects of dynamic compressive load on Collagen-based scaffolds seeded with Fibroblast-like synoviocytes. Tissue Eng 12(6):1527-1537, 2006.

- Glaeser JD, Salehi K, Kanim LE, NaPier Z, Kropf MA, Cuellar J, Sheyn D, Bae HW. Treatment with the NF?B inhibitor reduces overloading-induced MMP expression in human nucleus pulposus cells. The Spine Journal 17(10):S127,2017.

- Gosset M, Berenbaum F, Levy A, Pigenet A, Thirion S, Saffar JL, Jacques C. Prostaglandin E2 synthesis in cartilage explants under compression: mPGES-1 is a mechanosensitive gene. Arthritis Research & Therapy 8:R135,2006.

- Graff RD, Lazarowski ER, Banes AJ, Lee GM. ATP release by mechanically loaded porcine chondrons in pellet culture. Arthritis Rheum 43(7):1571-1579, 2000.

Flexcell細(xì)胞加壓培養(yǎng)系統(tǒng)

美國(guó)flexcell:體外細(xì)胞應(yīng)力刺激培養(yǎng)金標(biāo)準(zhǔn)

l  歷史悠久、成功使用案例和文獻(xiàn)多、無(wú)技術(shù)和使用風(fēng)險(xiǎn)：30多年歷史，6000+文獻(xiàn)，1300+實(shí)驗(yàn)室使用，國(guó)內(nèi)200+單位使用

l  真空動(dòng)力柔性基底膜細(xì)胞應(yīng)力應(yīng)變和三維細(xì)胞接種凝膠應(yīng)力應(yīng)變

l  不同基底剛度牽張：2019年研制了基底剛度1-60kPa可調(diào)控牽張拉伸培養(yǎng)皿和多孔板。

l  加載力學(xué)類型全面：牽張、壓力、流體剪切應(yīng)力

l  受力均勻、培養(yǎng)效果好：真空氣體牽張、壓縮刺激方式

l  不同基底剛度模量力學(xué)環(huán)境裝置的廠家，2019進(jìn)一步研制了 “更軟”基底剛度可調(diào)節(jié)可牽張培養(yǎng)裝置：

     0.1-80kPa可調(diào)控牽張拉伸培養(yǎng)皿和多孔板，避免細(xì)胞在體外培養(yǎng)受到“硬度”沖擊

l  模擬任意波形：動(dòng)靜、靜態(tài)以及自定義波形

l  細(xì)胞量大，便于后期分析：支持四個(gè)24孔或6孔牽張拉伸

l  即用型國(guó)際標(biāo)準(zhǔn)化的各種蛋白預(yù)先包被應(yīng)力刺激培養(yǎng)耗材

l  適用各種形態(tài)細(xì)胞組織樣品：?jiǎn)螌蛹?xì)胞、3D細(xì)胞構(gòu)建體、組織或3D聚集體

l  多模態(tài)刺激：靜態(tài)刺激、周期性動(dòng)態(tài)刺激、動(dòng)靜態(tài)對(duì)照、單軸向或雙軸向（等邊軸）牽張刺激

l  的可以動(dòng)靜態(tài)對(duì)照的廠家：可使同一塊壓力或牽張培養(yǎng)板部分孔受力。

l  的倒置和正置顯微鏡系統(tǒng)都兼容的廠家： 受力同時(shí)可實(shí)時(shí)觀察

l  整合柔性基底拓?fù)湮D案和微通道, 方便高級(jí)實(shí)驗(yàn)?zāi)Ｐ驮O(shè)計(jì)

l  整合力電刺激偶聯(lián)，方便高級(jí)實(shí)驗(yàn)?zāi)Ｐ驮O(shè)計(jì)

l  牽張、流體剪切多力混合同時(shí)加載，方便高級(jí)實(shí)驗(yàn)?zāi)Ｐ驮O(shè)計(jì)

l  使用Flexcell® Transwell Holder三維細(xì)胞（或者）拉力或壓力共培養(yǎng)

l  設(shè)備模塊化設(shè)計(jì)牽張、壓縮、流體剪切可以任意組合，緊湊大氣。