含有 GlutaMAX 添加劑的 Gibco RPMI 1640 培養(yǎng)基含有穩(wěn)定形式的 L-，在細(xì)胞培養(yǎng)時不會自發(fā)降解，可以防止氨沉積，改善細(xì)胞健康狀況并提高細(xì)胞活性。

RPMI 1640 培養(yǎng)基含有還原劑、、12 以及 PABA，這些維生素是 Eagle 必需培養(yǎng)基和 Dulbecco 改良 Eagle 培養(yǎng)基所不具備的。此外，RPMI 1640 培養(yǎng)基含有高濃度維生素肌醇和。由于 RPMI 1640 培養(yǎng)基不包含蛋白質(zhì)、脂質(zhì)或生長因子，因此通常需要補(bǔ)充胎牛血清（FBS）。RPMI 1640 培養(yǎng)基使用的緩沖系統(tǒng)需要在5–10%的 CO₂ 環(huán)境條件下來維持生理 pH 值。

賽默飛世爾科技針對多種細(xì)胞培養(yǎng)應(yīng)用，對 RPMI 培養(yǎng)基組分進(jìn)行了大量的改良。

RPMI 1640 Medium (Roswell Park Memorial Institute 1640 Medium) was originally developed to culture human leukemic cells in suspension and as a monolayer. RPMI 1640 medium has since been found suitable for a variety of mammalian cells, including HeLa, Jurkat, MCF-7, PC12, PBMC, astrocytes, and carcinomas. We offer a variety of RPMI 1640 modifications for a range of cell culture applications. Find the right formulation using the media selector tool.

The complete formulation is available.

Using RPMI 1640 Medium
RPMI 1640 medium is unique from other media because it contains the reducing agent glutathione and high concentrations of vitamins. RPMI 1640 medium contains biotin, vitamin B₁₂, and PABA, which are not found in Eagle's Minimal Essential Medium or Dulbecco's Modified Eagle Medium. In addition, the vitamins inositol and choline are present in very high concentrations. RPMI 1640 medium with GlutaMAX™ supplement minimizes toxic ammonia build-up and improves cell viability and growth in an easy-to-use format. RPMI 1640 medium contains no proteins, lipids, or growth factors. Therefore, RPMI 1640 medium requires supplementation, commonly with 10% Fetal Bovine Serum (FBS). RPMI 1640 medium uses a sodium bicarbonate buffer system (2.0 g/L) and therefore requires a 5–10% CO₂ environment to maintain physiological pH.