產(chǎn)品名稱：pCMV-Gag-Pol Retroviral Vector    
產(chǎn)品價(jià)格：8498
產(chǎn)    地：cell biolabs
產(chǎn)品貨號(hào)：RV-111
產(chǎn)品規(guī)格：10 μg
產(chǎn)品說(shuō)明：
STORAGE: -20ºC
Overview
These vectors provide the structural genes required to package your recombinant retrovirus. Use these vectors along with a packaging cell line and a cloning / expression vector for retroviral production.
Background
Retroviruses are efficient tools for delivering heritable genes into the genome of dividing cells. Moloney Murine Leukemia Virus （MMLV）-based retroviral vector system is the most commonly used gene transfer vehicle. pCMV-Gag-Pol expresses the retroviral structure proteins under the control of the CMV immediate-early promoter. The gag region encodes genes which comprise the capsid proteins; the pol region encodes the reverse transcriptase and integrase proteins.
Retrovirus can be produced using one of the following methods:
1） Transfection of a retrovirus packaging cell line with a retrovirus expression vector. Packaging cell lines usually stably express gag, pol and env genes. For example, transfection of Plat-E packaging cell line （Cat. # RV-101） with a pMXs vector would produce an ecotropic retrovirus.
2） Cotransfection of a host cell with plasmids containing LTRs, Gag, Pol, Env. For example, cotransfection of 293RTV （Cat.# RV-100） with pMXs, pCMV-Gag-Pol （Cat. # RV-111） and pCMV-VSV-G （Cat. # RV-110） would produce VSVG-pseudotyped retrovirus.
Note: We recommend cotransfection of expression vector:gag-pol vector:envelope vector at the following plasmid ratios:
（a） For ecotropic or amphotropic retrovirus, 3:1:1
（b） For VSVG-pseudotyped retrovirus, 3:1:0.5
The pCMV-Gag-Pol vector contains the ampicillin-resistance gene for propagation and antibiotic selection in bacteria