產品名稱：ViraSafe™ Lentiviral Packaging System, Ecotropic
產品價格：10725
產    地：cell biolabs
產品貨號：VPK-205
產品規(guī)格：1 kit
其它產品：
ViraSafe™ Lentiviral Packaging System, Pantropic 10725 cell biolabs VPK-206 1 kit
產品說明：
Storage
Upon receipt, store all other kit components at -20ºC until their expiration dates.
Introduction
Lentivirus vector based on the human immunodeficiency virus-1 （HIV-1） has become a promising vector for gene transfer studies. The advantageous feature of lentivirus vector is the ability of gene transfer and integration into dividing and non-dividing cells. Lentivirus pseudotyped with the MLV ecotropic envelope glycoprotein will only transduce mouse and rat cells with high efficiency. Lentiviral vectors have been shown to deliver genes to neurons, lymphocytes and macrophages, cell types that previous retrovirus vectors could not be used. Lentiviral vectors have also proven to be effective in transducing brain, liver, muscle, and retina in vivo without toxicity or immune responses. Recently, the lentivirus system is widely used to integrate siRNA efficiently in a wide variety of cell lines and primary cells both in vitro and in vivo.
Lentivirus particles are produced from 293T cells through transient transfection of plasmids that encode for the components of the virion （Figure 1）. Due to safety concerns regarding the infectious nature of HIV-1, recent lentiviral packaging systems have separated the viral components into 3 or 4 plasmids. However, these systems still present a small chance of generating replication-competent lentivirus upon recombination. In addition, most commercial lentiviral packaging systems provide plasmids containing the viral structure proteins in a premixed formulation, making it nearly impossible to optimize the ratio of the various plasmids for your particular experiment and host cell.
Cell Biolabs’ ViraSafe™ Lentiviral Packaging System provides a much safer method to package lentivirus, while still providing high viral titers. In addition, each plasmid is provided separay rather than in a packaging mixture. This allows you the flexibility to amplify individual plasmids and optimize the ratio of plasmids for your experiment.
Key Features of ViraSafe™ Lentiviral Packaging System:
1. Packaging Plasmids: Improve the packaging plasmid to increase performance and reduce the likelihood of recombination between vector components.
a. Minimize HIV sequences – no accessory proteins, Tat or Rev, or LTRs
b. Prevent overlap with vector SM by codon wobbling Gag sequences
c. Boost particle production by incorporating adenovirus VAI element
2. Flexible: All vectors including packaging vectors are provided separay to allow end-user to optimize the vector ratio for maximal lentivirus production.