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化工儀器網(wǎng)>產(chǎn)品展廳>試劑標(biāo)物>生化試劑>抗體/抗原> BD Pharm Lyse™ Lysing Buffer ...

BD Pharm Lyse™ Lysing Buffer 555899

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  • 公司名稱 江蘇省科學(xué)器材有限公司
  • 品牌 BD/美國(guó)
  • 型號(hào)
  • 產(chǎn)地 美國(guó)
  • 廠商性質(zhì) 代理商
  • 更新時(shí)間 2025/4/27 15:17:53
  • 訪問(wèn)次數(shù) 282
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BD試劑555899BD抗體試劑BD流式試劑

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江蘇省科學(xué)器材有限公司成立于1963年,總部位于江蘇省南京市,是一個(gè)有著超60經(jīng)營(yíng)實(shí)驗(yàn)室設(shè)備/耗材及分析儀器的綜合供應(yīng)商,公司具有自營(yíng)和代理各類商品和技術(shù)的進(jìn)出口權(quán)。

 

2005年公司根據(jù)省政府有關(guān)文件,在省科技廳的直接領(lǐng)導(dǎo)下,整體改制成為股份制公司。

 

    長(zhǎng)期以來(lái),江蘇科器服務(wù)超過(guò)2000家客戶,1000多個(gè)實(shí)驗(yàn)室,1500多家二級(jí)經(jīng)銷商。服務(wù)客戶包括(不限于)高??蒲性核⑨t(yī)療機(jī)構(gòu)、各政府實(shí)驗(yàn)室、第三方檢測(cè)機(jī)構(gòu)及各類企業(yè),是江蘇省內(nèi)較大的實(shí)驗(yàn)室設(shè)備、耗材試劑的集成商。

    

    同時(shí)江蘇科器還榮獲中國(guó)科學(xué)器材產(chǎn)銷聯(lián)合會(huì)副理事長(zhǎng)單位,榮獲AAA誠(chéng)信供應(yīng)商、AAA重質(zhì)量守信用企業(yè)、AAA信用等級(jí)證書、AAA資信等級(jí)證書、AAA重服務(wù)守信用企業(yè)、AAA誠(chéng)信經(jīng)營(yíng)示范單位、AAA重合同守信用單位、ISO 9001:2015質(zhì)量管理體系認(rèn)證證書、ISO 14001:2015環(huán)境管理體系認(rèn)證證書、ISO 45001:2018職業(yè)健康安全管理體系認(rèn)證證書等多項(xiàng)榮譽(yù)證書。

    

江蘇科器主要簽約代理的品牌有:島津SHIMADZU 、牛津儀器OXFORD 貝克曼庫(kù)爾特BECKMAN COULTER﹑梅特勒托利多METTLER TOLEDO艾卡IKA﹑美墨爾特Memmert﹑艾本德 Eppendorf、 蘇凈安泰、上海知楚、上海一恒、北京白洋、廈門國(guó)儀、保定蘭格、上海磐麥等國(guó)內(nèi)外品牌。    

 

另外,江蘇科器擁有十六名應(yīng)&售后工程師團(tuán)隊(duì),可為廣大客戶提供實(shí)驗(yàn)室設(shè)備的技術(shù)培訓(xùn)、維修維護(hù)保養(yǎng)、整體搬遷等服務(wù)。提供至客戶多方位、 深層次的服務(wù)與合作。


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供貨周期 兩周 規(guī)格 100ml
貨號(hào) 555899

BD Pharm Lyse™ Lysing Buffer 555899



BD Pharm Lyse™ lysing solution is a buffered, concentrated (10X) ammonium chloride-based lysing reagent. When diluted to a 1X concentration and used as recommended, BD Pharm Lyse™ lyses red blood cells following monoclonal antibody staining. The lysing solution results in good light scatter separation of lymphocytes and red blood cell debris when analyzed by flow cytometry.  BD Pharm Lyse™ does not contain a fixative agent, so leukocytes remain viable after red blood cell lysis.


Brand:BD Pharm Lyse™

Regulatory Status:RUO

Application:Flow cytometry (Routinely Tested)


BD Pharm Lyse™ Lysing Buffer 555899

準(zhǔn)備和存儲(chǔ)

Store undiluted at 4°C.

1X diluted solutions may be stored at 4° C for up to 30 days.



推薦的實(shí)驗(yàn)流程

Preparation of 1X lysing solution:  Dilute the 10X concentrate 1:10 with distilled water. The pH of the 1X solution should fall within the range of pH 7.1-7.4.  Adjust the pH if necessary. Warm the 1X solution to room temperature prior to use. 100 ml of 10X concentrate will yield a quantity of 1X solution that is sufficient to lyse 500 samples.


Lysing procedure:

Note: The following procedure is only applied to human whole blood red blood cell lysis. Since applications vary, for other samples such as mouse spleen red blood cell lysis (lysing incubation time up to 3 minutes at 37°C), bone marrow red blood cell lysis, each investigator should optimize the condition to obtain optimal results.

1. Add 2.0 ml  of 1X lysing solution to each tube containing up to 200 µl of a whole blood plus monoclonal antibody mixture.

2. Gently vortex each tube immediately after adding the lysing solution.

3. Incubate at room temperature, protected from light, for 15 minutes.

4. Centrifuge 200 X g for 5 minutes.

5. Carefully aspirate supernatent, without disturbing pellet.

6. Add 2.0 ml 1X PBS containing 1% heat-inactivated fetal bovine serum and 0.1% sodium azide (PBS-FBS).

7. Centrifuge at 200 X g for 5 minutes.

8. Carefully aspirate supernatent, without disturbing pellet.

9. Resuspend pellet in 0.5 ml PBS-FBS or a fixative such as 2% formaldehyde for flow cytometric analysis.


Troubleshooting: Incomplete lysis may occur for several reasons: (1) The age of the specimen may affect red cell lysis, (2) The 1X lysing solution may not have been warmed to room temperature, or (3) After adding the lysing solution, the samples may not have been vortexed sufficiently. If incomplete lysis occurs (recognized by the presence of an excessive amount of visible red blood cells in the final, washed cell suspension), repeat lysing procedure. However, lysing more than two times is not recommended.





BD Pharm Lyse™ Lysing Buffer 555899




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